Manual for GBRED

Query Results | Graph table | Repetitive element | Tutorial


GBRED (Gene-Based Repetitive Element Database) is a dedicated database containing repetitive element information which focus on the sequences flanking all human genes, such as 5' untranslated region (5'UTR), and 3' untranslated region (3'UTR). You can click the query button to search the information of repetitive elements surrounding specific genes. If you want to analyze your own sequences, you can click the “RepeatMasker” button to link to RepeatMasker serve which provided by NCKU.

Query result
After selecting options and clicking the search button, genes which match to your selection would be showed in a list. This gene list contains gene id, gene symbol, and gene description, helping you to choose the gene of interest. If you do not select the chromosomal icon in the query page, the gene list might show the chromosomal location of each gene. Click the gene id in the list could see the detail information of selected gene, including

Chromosome Indicate the chromosomal location of selected gene
Gene id The accession number approved by Ensembl and has a hyperlink to Ensembl webpage
Gene symbol The official symbol approved by the UCL/HGNC Human Gene NomenClature database
Description General description of gene in Ensemb
Strand Indicate the gene is located in plus or minus strand
Biotype Indicate the gene classes in Ensembl
Gene location The detail chromosome location of selected gene
Transcript List all transcripts of the gene

 


 

Graph table
Click the transcript would show the information of repetitive elements located at surrounding region of selected transcript. First, you would see a legend which illustrates the graph of repetitive elements. Below legend, there are some graphs and tables to show information of repetitive elements in different regions. These graphs would show the transcription start site and transcription end site that you could understand the relative location of these repetitive elements and while you click the sections on the graph, there will be a window to show what these sections mean. The table under graph could demonstrate the detail information about each repetitive element, including:

Query Region The section in each region match with the consensus of repetitive element in Repbase
(ex. flank_u5000, 5UTR, 3UTR, flank_d5000)
Match Type The section match to the plus or minus strand of repetitive element consensus. "+" means plus strand; "C" means complementary strand

Repeat Name The specific name of repetitive element defined by Repbase
Repeat Class Class of repetitive element defined by Repbase
Hit Region The section in consensus of repetitive element which match to the section of each region

 


 

Classification of repetitive element

SINEs (Short interspersed elements)
Class family Description
SINE/Alu SINE, Alu family
SINE/MIR SINE, MIR family
LINEs (Long interspersed elements)
Class family Description
LINE/CR1 CR1 (chicken repeat 1) – LINE
LINE/L1 L1 repeat of LINE
LINE/L2 L2 repeat of LINE
LINE/RTE RTE (RNA Transport Element ) -type non-LTR retrotransposon
LTR (Long termianl repeat) retroelements
Class family Description
LTR/ERV LTR, Endogenous Retrovirus (ERV)class
LTR/ERV1 LTR, ERV1 class
LTR/ERVK LLTR, human endogenous retrovirus K (HERV-K)
LTR/ERVL LTR, ERVL class
LTR/MaLR Mammalian apparent LTR retrotransposons (MaLR) family
DNA elements
Class family Description
DNA Including medium reiteration frequency repeat 53 (Mer53), Mer103, Mer105
DNA/AcHobo DNA transposon, AcHobo family
DNA/hAT DNA transposon, hAT (hobo, Ac and Tam3) family
DNA/Mariner DNA transposon, Mariner family
DNA/MER1_type DNA transposon, MER1 group
DNA/MER1_type? DNA transposon, MER1 group?
DNA/MER2_type DNA transposon, MER2 group
DNA/Merlin DNA transposon, Merlin group
DNA/MuDR ?MuDR-type DNA transposon element
DNA/PiggyBac DNA transposon, PiggyBac group
DNA/Tc2 DNA transposon, Tc2 family
DNA/Tip100 DNA transposon, Tip100 family
Small RNA classes
Class family Description
RNA Pseudogene; RNA group
rRNA Pseudogene; 5S ribosomal RNA; ribosomal RNA
scRNA Pseudogenes; scRNA group
snRNA Pseudogenes; snRNA group
srpRNA Pseudogenes; srpRNA group
tRNA Pseudogene; tRNA group
Satellites
Class family Description
Satellite Satellite
Satellite/acro Acromeric satellite
Satellite/centr Centromeric satellite DNA
Satellite/telo Telomeric satellite
Simple repeats
Class family Description
Simple_repeat Repeats of 1–6 nucleotides
Low complexity
Class family Description
Low_complexity Including A-rich, AT-rich, C-rich, CT-rich, G-rich, GA-rich, GC-rich, polypurine, polypyrimidine and T-rich repeats